In this study,  we show that varying the assistive knee torque provided by a powered knee prosthesis significantly improved weight-bearing symmetry, effort, and speed during the stand-up movement in eight above-knee amputees. Specifically, we observed improvements in the index of asymmetry of the vertical ground reaction force at the point approximating maximum vertical center of mass acceleration, the integral of the intact vastus medialis activation measured using electromyography, and the stand-up duration compared to the passive prosthesis. These results show that powered prostheses can improve functional mobility, potentially increasing quality of life for millions of people living with above-knee amputations. The manuscript can be downloaded freely at this link: Effect of Increasing Assistance from a Powered Prosthesis on Weight-Bearing Symmetry, Effort, and Speed during Stand-Up in Individuals with Above-Knee Amputation | IEEE Journals & Magazine | IEEE Xplore